Mesenchymal stem cell response to growth factor treatment and low oxygen tension in 3-dimensional construct environment

摘要: Introduction: cell-based tissue engineering strategies using human mesenchymal stem cells (hMSCs) may help to augment tendon healing. To further investigate the in-vitro behavior of this cell population, we investigated low oxygen culture levels, and growth factor supplementation their effect on expression extracellular proteins proliferation. Materials methods: bone marrow aspirate (BMA) was harvested during arthroscopic rotator cuff repair. Characterized hMSCs derived from BMA were incorporated into 3-dimensional engineered constructs (TECs). TECs analyzed by frozen sections with immunohistochemistry for density, collagen I III expression. Results: stimulation increased density within TECs. Low addition factors media demonstrated an increase in expression, both ambient conditions conditions. Conclusion: TGFβ3 a positive number, type 3D environments