Infectious clones of Chikungunya virus (La Réunion isolate) for vector competence studies.
作者： Konstantin Tsetsarkin , Stephen Higgs , Charles E. McGee , Xavier De Lamballerie , Remi N. Charrel
关键词: Genotype 、 Outbreak 、 Aedes aegypti 、 Virus 、 Biology 、 Aedes albopictus 、 Alphavirus infection 、 Chikungunya 、 Virology 、 Vector (epidemiology)
摘要: The recent outbreak of Chikungunya virus (CHIKV) on several islands in the Indian Ocean and India has focused attention this reemerging highlighted need for development new tools to study vector-virus-host interactions. We have constructed characterized, cell culture, Aedes aegypti Ae. albopictus mosquitoes, infectious cDNA clones CHIKV using a isolate from La Reunion Island. Comparison growth kinetics infection rates viral strain LR2006 OPY1 (CHIKV-LR) full-length clone (CHIKV-LR ic) indicate that retained phenotypes original isolate. Infectious express green fluorescent protein (GFP) were also produced characterized culture mosquitoes. CHIKV-LR 5'GFP infected mosquitoes at similar rate full length clone. 3'GFP only rates. these authentic will enable targeted studies molecular determinants infection, pathogenesis transmission competence by
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