Systems biology reveals NR2F6 and TGFB1 as key regulators of feed efficiency in beef cattle
作者： Pâmela A. Alexandre , Marina Naval-Sanchez , Laercio R. Porto-Neto , José Bento S. Ferraz , Antonio Reverter
DOI: 10.1101/360396
关键词: Single-nucleotide polymorphism 、 Systems biology 、 Biology 、 Regulator gene 、 Candidate gene 、 Gene 、 Computational biology 、 Phenotype 、 Beef cattle 、 Feed conversion ratio
摘要: Systems biology approaches are used as strategy to uncover tissue-specific perturbations and regulatory genes related complex phenotypes. We applied this approach study feed efficiency (FE) in beef cattle, an important trait both economically environmentally. Poly-A selected RNA of five tissues (adrenal gland, hypothalamus, liver, skeletal muscle pituitary) eighteen young bulls, for high low FE, were sequenced (100bp, pared-end). From the 17,354 expressed genes, 1,317 prioritized by selection categories (differentially expressed, harbouring SNPs associated with tissue-specific, secreted plasma key regulators) network construction. NR2F6 TGFB identified validated motif discovery regulators hepatic inflammatory response tissue development, respectively, two biological processes demonstrated be FE. Moreover, we indicated potential biomarkers FE which hormonal control metabolism sexual maturity. By using robust methodologies validation strategies, confirmed main Bos indicus candidate or superior animals.
sci-hub.st 参考文章(94)
Robert M. Koch, L. A. Swiger, Doyle Chambers, K. E. Gregory, Efficiency of Feed Use in Beef Cattle Journal of Animal Science. ,vol. 22, pp. 486- 494 ,(1963) , 10.2527/JAS1963.222486X
J A Archer, P F Arthur, R M Herd, P F Parnell, W S Pitchford, Optimum postweaning test for measurement of growth rate, feed intake, and feed efficiency in British breed cattle Journal of Animal Science. ,vol. 75, pp. 2024- 2032 ,(1997) , 10.2527/1997.7582024X
Phillip R. Myer, Timothy P. L. Smith, James E. Wells, Larry A. Kuehn, Harvey C. Freetly, Rumen Microbiome from Steers Differing in Feed Efficiency PLOS ONE. ,vol. 10, pp. e0129174- ,(2015) , 10.1371/JOURNAL.PONE.0129174
Bernhard Schmierer, Caroline S. Hill, TGFβ–SMAD signal transduction: molecular specificity and functional flexibility Nature Reviews Molecular Cell Biology. ,vol. 8, pp. 970- 982 ,(2007) , 10.1038/NRM2297
Lisa A. Levin, Guillermo F. Mendoza, Benjamin M. Grupe, Jennifer P. Gonzalez, Brittany Jellison, Greg Rouse, Andrew R. Thurber, Anders Waren, Biodiversity on the Rocks: Macrofauna Inhabiting Authigenic Carbonate at Costa Rica Methane Seeps. PLOS ONE. ,vol. 10, ,(2015) , 10.1371/JOURNAL.PONE.0131080
Marta Giralt, Aleix Gavaldà-Navarro, Francesc Villarroya, Fibroblast growth factor-21, energy balance and obesity Molecular and Cellular Endocrinology. ,vol. 418, pp. 66- 73 ,(2015) , 10.1016/J.MCE.2015.09.018
A.B.P. Fontoura, Y.R. Montanholi, M. Diel de Amorim, R.A. Foster, T. Chenier, S.P. Miller, Associations between feed efficiency, sexual maturity and fertility-related measures in young beef bulls. Animal. ,vol. 10, pp. 96- 105 ,(2016) , 10.1017/S1751731115001925
Brian Karisa, Stephen Moore, Graham Plastow, Analysis of biological networks and biological pathways associated with residual feed intake in beef cattle Animal Science Journal. ,vol. 85, pp. 374- 387 ,(2014) , 10.1111/ASJ.12159
Alberto de la Fuente, From ‘differential expression’ to ‘differential networking’ – identification of dysfunctional regulatory networks in diseases Trends in Genetics. ,vol. 26, pp. 326- 333 ,(2010) , 10.1016/J.TIG.2010.05.001
R. M. Herd, V. H. Oddy, E. C. Richardson, Biological basis for variation in residual feed intake in beef cattle 1: Review of potential mechanisms Animal Production Science. ,vol. 44, pp. 423- 430 ,(2004) , 10.1071/EA02220