A GC/MS method for determination of succinylacetone in Arabidopsis thaliana.

摘要: Succinylacetone was known to be a toxic metabolite of tyrosine in human and animals caused by blockage the final step degradation pathway, but its existence plant unclear though metabolic disturbance also found plant. A GC-MS method for determination succinylacetone Arabidopsis thaliana developed first time. Both oximation silylation were applied derivation procedure, low-temperature condition before completion necessary obtain good linearity calibration curve due thermolability succinylacetone. The specific chromatogram pattern formed four isomers derivatives provided helpful feature identification. detection limit proposed 0.25 ppm A. thaliana. recoveries between 95.4 109.3 % with coefficient variation ranging from 4.36 7.81 intra-day assays 6.47 8.52 inter-day assays. Application wild-type short-day sensitive cell death 1 mutant represented an obvious correlation measured amount wilting symptom, suggesting could powerful tool further study on toxicology catabolism plants.