Antigen stimulation of peripheral blood mononuclear cells from Mycobacterium bovis infected cattle yields evidence for a novel gene expression program

摘要: Bovine tuberculosis (BTB) caused by Mycobacterium bovis continues to cause substantial losses global agriculture and has significant repercussions for human health. The advent of high throughput genomics facilitated large scale gene expression analyses that present a novel opportunity revealing the molecular mechanisms underlying mycobacterial infection. Using this approach, we have previously shown innate immune genes in peripheral blood mononuclear cells (PBMC) from BTB-infected animals are repressed vivo absence exogenous antigen stimulation. In study, hypothesized PBMC cattle would display distinct program resulting exposure M. bovis. A functional approach was used examine response (n = 6) healthy control stimulation with bovine tuberculin (purified protein derivative – PPD-b) vitro. were harvested before, at 3 h 12 post vitro tuberculin. Gene changes catalogued within each group using reference hybridization design targeted immunospecific cDNA microarray platform (BOTL-5) 4,800 spot features representing 1,391 genes. 250 significantly differentially expressed post-stimulation contrasting only 88 non-infected (P ≤ 0.05). At post-stimulation, 56 80 both groups respectively. results provided evidence proinflammatory profile Furthermore, common panel eighteen genes, including transcription factors opposite directions groups. Real-time quantitative reverse PCR (qRT-PCR) demonstrated many components TLR pathway cytokines 8) versus after exhibit different transcriptional profiles compared stimulation, providing due exposure.