Identifying sex in non-fertile individuals of the moss Drepanocladus turgescens (Bryophyta: Amblystegiaceae) using a novel molecular approach.

摘要: Sex identification before sexual maturity is notoriously difficult in plants with separate sexes, but crucial to address many life history related issues. To study the performance of two sexes rarely sexually reproducing dioecious moss Drepanocladus turgescens a molecular sex marker needed. The female-targeting previously developed for D. trifarius and lycopodioides amplifies few males, which can thus not be distinguished from females. In significant addition earlier method we sequenced portion successfully amplified by primers PT-3f PT-3r six females three males. Differences between males were revealed at five sequence positions. Examination total fourteen seven amplifying confirm that such differ consistently these usefulness previous protocol extended another step where sex-correlated region sequenced.