Interaction of hexokinase with the outer mitochondrial membrane and a hydrophobic matrix.

摘要: The major portion of rat brain hexokinase (HK type I) is bound to the outer membrane mitochondria and glucose-6-phosphate (G6P) can release enzyme. In an attempt look at ‘hydrophobic’ component binding, interaction enzyme with a purely hydrophobic matrix, palmityl-substituted Sepharose-4B (Sepharose-lipid) was investigated. Hexokinase readily this matrix retention its catalytic activity. Glucose-6-phosphate which has releasing effect on mitochondrially enzyme, enhanced binding matrix. Chymotrypsin treatment causes loss mitochondria, also results in adsorption thus demonstrating that ‘hydrophobic tail’ present N-terminal end essential for both cases. Data presented provide some new information relevant understanding how interacts natural mitochondrion.