Neurite development in vitro. I. The effects of adenosine 3′5′‐cyclic monophosphate (cyclic AMP)

摘要: Elevated levels of 3′5′ adenosine monophosphate (cyclic AMP) stimulate a wide variety cellular events including aggregation, differentiation, morphological expression, pigment migration, and secretion. The role cyclic AMP in these prompted our present study embryonic chick dorsal root ganglia. Test substances were applied to cultures during the routine feeding procedure. Their development was quantitatively evaluated on basis explant size, length glial-like outgrowth, distribution growth, neurite number, length, diameter, degree arborization. These parameters all shown be independent each other. high variability vitro necessitated use over 100 per treatment group. Cultures treated with 5′ exhibited no significant differences from controls. Those AMP, dibutyryl or Nerve Growth Factor (NGF) statistically increases area number neurites culture, diameters, lengths, growth promoting activity NGF greater than those AMP. Electron microscopic shows formed under influence its derivative resemble grown NGF. studies suggest possibility that stimulates by mediating process microtubule (MT) assembly. They further prompt us speculate one way enhances is stimulating MT assembly via “Second Messenger System”.