PROTEOMICS OF THE RED ALGA, GRACILARIA CHANGII (GRACILARIALES, RHODOPHYTA)1
作者： Pooi-Fong Wong , Li-Jing Tan , Hannah Nawi , Sazaly AbuBakar
DOI: 10.1111/J.1529-8817.2006.00182.X
关键词: Trichloroacetic acid 、 Biochemistry 、 Botany 、 Biology 、 Gel electrophoresis 、 Gracilariales 、 Proteome 、 Tris 、 Protein purification 、 Proteomics 、 Lysis 、 Plant science 、 Aquatic science
摘要: The application of proteomics in alga research is still quite limited. present report describes the establishment proteome a red economic importance, Gracilaria changii (Xia et Abbott) Abbott, Zhang Xia. Initially, four protein extraction methods including direct precipitation by trichloroacetic acid/acetone, lysis using urea buffer, Tris and phenol/chloroform were compared for their suitability to generate G. proteins two-dimensional gel electrophoresis (2-DE). method gave best 2-DE resolution proteins. Using these gels mass spectrometry, several pigment proteins, metabolic enzymes, ion transporters identified. These findings highlight potential proteomic approaches investigation function.
sci-hub.se 参考文章(27)
Mentz Indergaard, The Aquatic Resource Springer, Boston, MA. pp. 137- 168 ,(1983) , 10.1007/978-1-4757-0833-2_8
David N. Perkins, Darryl J. C. Pappin, David M. Creasy, John S. Cottrell, Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. ,vol. 20, pp. 3551- 3567 ,(1999) , 10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2
Wenzhu Zhang, Brian T. Chait, ProFound: An Expert System for Protein Identification Using Mass Spectrometric Peptide Mapping Information Analytical Chemistry. ,vol. 72, pp. 2482- 2489 ,(2000) , 10.1021/AC991363O
Robert van Lis, Diego González-Halphen, Ariane Atteia, Divergence of the mitochondrial electron transport chains from the green alga Chlamydomonas reinhardtii and its colorless close relative Polytomella sp. Biochimica et Biophysica Acta. ,vol. 1708, pp. 23- 34 ,(2005) , 10.1016/J.BBABIO.2004.12.010
Gurvan Michel, William Helbert, Richard Kahn, Otto Dideberg, Bernard Kloareg, The structural bases of the processive degradation of iota-carrageenan, a main cell wall polysaccharide of red algae. Journal of Molecular Biology. ,vol. 334, pp. 421- 433 ,(2003) , 10.1016/J.JMB.2003.09.056
Mohd Hani Norziah, Chio Yen Ching, Nutritional composition of edible seaweed Gracilaria changgi Food Chemistry. ,vol. 68, pp. 69- 76 ,(2000) , 10.1016/S0308-8146(99)00161-2
D. F. KAPRAUN, Nuclear DNA content estimates in multicellular green, red and brown algae: phylogenetic considerations. Annals of Botany. ,vol. 95, pp. 7- 44 ,(2005) , 10.1093/AOB/MCI002
Jeri Welsh Higginbotham, J. Stephen C. Smith, Oscar S. Smith, Quantitative analysis of two-dimensional protein profiles of inbred lines of maize (Zea mays L.). Electrophoresis. ,vol. 12, pp. 425- 431 ,(1991) , 10.1002/ELPS.1150120608
A. Koller, M. P. Washburn, B. M. Lange, N. L. Andon, C. Deciu, P. A. Haynes, L. Hays, D. Schieltz, R. Ulaszek, J. Wei, D. Wolters, J. R. Yates, Proteomic survey of metabolic pathways in rice Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 11969- 11974 ,(2002) , 10.1073/PNAS.172183199
Valérie Méchin, Thierry Balliau, Sophie Château-Joubert, Marlène Davanture, Olivier Langella, Luc Négroni, Jean-Louis Prioul, Claudine Thévenot, Michel Zivy, Catherine Damerval, A two-dimensional proteome map of maize endosperm. Phytochemistry. ,vol. 65, pp. 1609- 1618 ,(2004) , 10.1016/J.PHYTOCHEM.2004.04.035