Binding of Human Alpha-Interferons to Natural Killer Cells

摘要: ABSTRACT It has previously been shown that recombinant DNA-derived human leukocyte interferon IFN-αJ, which is active on cells in antiviral and antiproliferative assays, substantially lacks the ability of other purified IFN (e.g., IFN-αA) to enhance natural killer (NK) activity. IFN-αJ can, however, block boosting NK activity by IFN-αA, suggesting can occupy IFN-α receptor cells.(1) We demonstrate here directly bind compete with [125I]IFN-αA for binding sites. The equilibrium dissociation constant (Kd) no more than 20– 30 times greater IFN-αA. relative Kd values IFN-αA are similar when measured Daudi cells, where both IFN-αs have potent effects. This suggests difference terms constants does not explain inactivity following a 2-h incubation.