Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus.

摘要: The organization of viral genes within the 3.7-kilobase-pair HindIII-K/EcoRI-S region Autographa californica nuclear polyhedrosis virus genome (85 to 88 map units) was determined by using a combination nucleotide sequencing, transcriptional mapping, and in vitro translation hybrid selected RNA. Two nonoverlapping genes, extending opposite directions encoding polypeptides with molecular weights 35,000 94,000 (35K 94K polypeptides), were identified. Unspliced, messenger-active RNAs transcribed from both early (2 h) after infection. Indicative immediate-early transcription divergent unaffected protein synthesis inhibitor, cycloheximide. Late infection, abundant promoters located at least 2.5 kilobase pairs upstream gene 35K polypeptide. These transcripts completely overlapped polypeptide but apparently lacked protein-coding potential, suggesting that may play role suppression expression.