The opine synthase genes carried by Ti plasmids contain all signals necessary for expression in plants

摘要: Signals necessary for in vivo expression of Ti plasmid T-DNA-encoded octopine and nopaline synthase genes were studied crown gall tumors by constructing mutated carrying various lengths sequences upstream the 5' initiation site their mRNAs. Deletions position -294 did not interfere with gene while those extending -170 greatly reduced expression. The estimated size promoter is therefore 295 bp. maximal length involved 261 Our results also demonstrated that plasmid-derived contain all signals essential opine plants. Expression these genes, therefore, independent direct vicinity plant DNA activated formation T-DNA border junction.