Acid‐sensing ion channel 1a is involved in acid‐induced osteoclastogenesis by regulating activation of the transcription factor NFATc1

摘要: It has been known that osteoclastogenesis is induced by extracellular acidosis-evoked the rise of intracellular calcium ([Ca2+]i), which regulate activation transcription factor nuclear activated T cells c1 (NFATc1). However, acid-sensing ion channels (ASICs) involved remain largely unknown. Here, we show ASIC1a, ASIC1b, ASIC2a, and ASIC3 are expressed in rat osteoclasts, only ASIC1a highly upregulated response to acidosis. Both ASIC1a-specific blocker PcTX1 specific siRNA significantly reduce this increase acid-induced [Ca2+]i translocation NFATc1, inhibit osteoclast differentiation bone resorption. These findings ASIC1a-mediated entry plays a critical role regulating NFATc1.