Isolation and Characterization of Broad Spectrum Coaggregating Bacteria from Different Water Systems for Potential Use in Bioaugmentation

摘要: The bridging bacteria with broad-spectrum coaggregation ability play an important role during multispecies-biofilm development. In this study, through a visual and semi-quantitative assay, twenty-two bacterial strains aggregation were obtained from 8 different water environments, these assigned to 7 genera according their 16S rDNA they Aeromonas, Bacillus, Comamonas, Exiguobacterium, Pseudomonas, Shewanella Comamonas. Furthermore, all possible 231 pairwise combinations among 22 explored for by spectrophotometric assay. Among strains, it was found that Bacillus cereus G5 megaterium T1 coaggregated themajority of assayed other 90.5% (19 21 strains) 76.2% respectively (17 at higher rates (A.I. greater than 50%), indicating have property. images coaggregates also confirmed the coexistence partner strains. Biofilm biomass development cocultured each its further evaluateded. results showed 15 when paired G5, developed biofilm monocultures. both fluorescence microscopy scanning electron (SEM) demonstrated A3-GFP (a 3,5-dinitrobenzoic acid-degrading strain, staining gfp),could develop typical spatial structure dual-species cocultured. These suggested bridging-bacteria broad spectrum coaggregating ability, such as G5,could mediate integration exogenous degrading into biofilms contribute bioaugmentation treatment.