Rapid screening of Mycobacterium tuberculosis for susceptibility to rifampicin and streptomycin.
作者： P. Kiepiela , N. G. Stoker , K. S. Bishop , R. McNerney , P. M. Nye
DOI:
关键词: Medicine 、 Molecular biology 、 Mycobacterium tuberculosis 、 Rifampicin 、 Streptomycin 、 Microbiology
摘要: OBJECTIF : Investiguer la detection rapide d'une tuberculose resistante aux medicaments grâce a technique genotypique inno-LiPA Rif TB associee un nouveau test de susceptibilite peu couteux base sur les bacteriophages. SCHEMA On compare performance du replication des pliages en microplaques (MPRA) 18 isolats patients suspects germes multiresistants (MDR) celle LiPA pratique directement echantillons d'expectoration. Les mutations gene rpoB identifiees par et qui conferent resistance rifampicine ont ete confirmees le sequencage l'ADN, alors que sensibilites methode proportions BACTEC. L'on inclus pour comparaison 19 supplementaires subissant depistage routine sensibilite streptomycine. RESULTATS La determinee dans 17 (94,4%) d'expectoration testes LiPA. correlation entre MPRA, methodes moleculaires conventionnelles 100% rifampicine. Toutefois, resultat discordant trouve ce concerne streptomycine isolat sensible s'est avere resistant 2 μg/ml MPRA. De maniere similaire, initialement MPRA lors repetitif, accord avec conventionnelle. CONCLUSION: permet une l'infection resistants medicaments, fournit simple n'exigeant pas technologie avancee, peut favoriser son application pays faibles revenus.
cabdirect.org参考文章(25)
Tohru Tokunaga, Margret I. Sellers, Streptomycin Induction of Premature Lysis of Bacteriophage-Infected Mycobacteria Journal of Bacteriology. ,vol. 89, pp. 537- 538 ,(1965) , 10.1128/JB.89.2.537-538.1965
John D. H. Porter, Keith P. W. J. McAdam, Tuberculosis : back to the future Wiley. ,(1994)
Tadao Shimao, Chemotherapy of Drug-Resistant Tuberculosis In the Context of Developed and Developing Countries Mycobacteria. pp. 183- 208 ,(1998) , 10.1007/978-1-4615-7511-5_7
S B Walters, B A Hanna, TESTING OF SUSCEPTIBILITY OF MYCOBACTERIUM TUBERCULOSIS TO ISONIAZID AND RIFAMPIN BY MYCOBACTERIUM GROWTH INDICATOR TUBE METHOD Journal of Clinical Microbiology. ,vol. 34, pp. 1565- 1567 ,(1996) , 10.1128/JCM.34.6.1565-1567.1996
Pillai G, Anastasis D, Rambiritch, Abdool Karim Ss, A retrospective study of human immunodeficiency virus infection and drug-resistant tuberculosis in Durban, South Africa. International Journal of Tuberculosis and Lung Disease. ,vol. 1, pp. 220- ,(1997)
Zulema Reggiardo, Gardner Middlebrook, William D. Tigertt, Automatable radiometric detection of growth of Mycobacterium tuberculosis in selective media. The American review of respiratory disease. ,vol. 115, pp. 1066- 1069 ,(1977) , 10.1164/ARRD.1977.115.6.1066
T. J. Hellyer, T. W. Fletcher, J. H. Bates, W. W. Stead, G. L. Templeton, M. D. Cave, K. D. Eisenach, Strand Displacement Amplification and the Polymerase Chain Reaction for Monitoring Response to Treatment in Patients with Pulmonary Tuberculosis The Journal of Infectious Diseases. ,vol. 173, pp. 934- 941 ,(1996) , 10.1093/INFDIS/173.4.934
David L. Cohn, Flavia Bustreo, Mario C. Raviglione, Drug-Resistant Tuberculosis: Review of the Worldwide Situation and the WHO/IUATLD Global Surveillance Project Clinical Infectious Diseases. ,vol. 24, ,(1997) , 10.1093/CLINIDS/24.SUPPLEMENT_1.S121
Kathleen D. Eisenach, Mark D. Sifford, M. Donald Cave, Joseph H. Bates, Jack T. Crawford, Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. The American review of respiratory disease. ,vol. 144, pp. 1160- 1163 ,(1991) , 10.1164/AJRCCM/144.5.1160
A Telenti, P Imboden, F Marchesi, T Schmidheini, T Bodmer, Direct, automated detection of rifampin-resistant Mycobacterium tuberculosis by polymerase chain reaction and single-strand conformation polymorphism analysis. Antimicrobial Agents and Chemotherapy. ,vol. 37, pp. 2054- 2058 ,(1993) , 10.1128/AAC.37.10.2054