Actin dynamics at pointed ends regulates thin filament length in striated muscle
作者： Ryan Littlefield , Angels Almenar-Queralt , Velia M. Fowler
DOI: 10.1038/35078517
关键词: CapZ 、 Cell biology 、 Tropomodulin 、 Arp2/3 complex 、 MDia1 、 Actin remodeling of neurons 、 Myofilament 、 Biology 、 Actin remodeling 、 Microfilament
摘要: Regulation of actin dynamics at filament ends determines the organization and turnover cytoskeletal structures. In striated muscle, it is believed that tight capping fast-growing (barbed) by CapZ slow-growing (pointed) tropomodulin (Tmod) stabilizes uniform lengths (thin) filaments in myofibrils. Here we demonstrate for first time both Tmod are dynamic on basis rapid incorporation microinjected rhodamine-labelled (rho-actin) barbed pointed from photobleaching green fluorescent protein (GFP)-labelled Tmod. Unexpectedly, inhibition GFP-Tmod overexpression results shorter thin filaments, whereas cytochalasin D has no effect length. These data myofibrils relatively despite presence proteins, regulated assembly length filaments.
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