Screening Microalgal Cultures in Search of Microbial Exopolysaccharides with Potential as Adhesives

摘要: Nearly 800 cultures from the Commonwealth Scientific and Industrial Research Organisation (CSIRO) Collection of Living Microalgae (CCLM) were screened for exopolysaccharide (EPS) production by examining viscosity conditioned media supernatants. We first established dose-response relationships reference polysaccharides dissolved in microalgal growth media. Then, using 40 most viscous CCLM cultures, we confirmed that culture supernatants correlated well with their total sugar (and thus EPS) content. The medium highest (6.55 cP, equivalent to 1.16 g/L xanthan gum) was produced a non-axenic isolate cyanobacterium Microcystis aeruginosa f. flos-aquae. Two types bacteria, designated CSIRO501 (Gram-positive) CSIRO505 (Gram-negative), subcultured it. At 20 dry wt% sugar, an exopolymer preparation contained substantially more EPS than its counterparts or axenic cul...