Solid-phase enzyme-linked immunosorbent assay for hepatitis E virus IgG and IgM antibodies utilizing recombinant antigens and synthetic peptides.
作者： George J. Dawson , Kurt H. Chau , Carlos M. Cabal , Patrice O. Yarbough , Gregory R. Reyes
DOI: 10.1016/0166-0934(92)90180-L
关键词: Hepeviridae 、 Hepatitis E virus 、 Virology 、 Recombinant DNA 、 Antigen 、 Antibody 、 Serology 、 Viral disease 、 Biology 、 Outbreak
摘要: Abstract Four recombinant antigens representing two distinct antigenic domains from different strains of hepatitis E virus (HEV) were used individually to develop four ELISAs designed detect antibodies HEV. Both IgG and IgM class HEV detected in 7 8 pedigreed serum/plasma known outbreaks Mexico, Burma, Somalia Pakistan. In addition, specific HEV-antibodies cynomolgus macaques following inoculation with various strains. Anti-HEV was also 386 (2.1%) randomly selected American blood donors. Supplemental tests utilizing both synthetic peptides blocking assays provided additional serologic data confirming the presence anti-HEV. Similar prevalence studies on a limited number available sera other geographical regions (Alaska, Japan, Germany, New Zealand, Thailand Mexico) confirmed anti-HEV at least 1.1 7.6% specimens.
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