Phenotypic difference of normal plasma cells from mature myeloma cells

摘要: We have recently shown that two-color analysis with fluorescein isothiocyanate (FITC)-anti-CD38 antibody could clearly distinguish myeloma cells (plasma cells) from other hematopoietic in the bone marrow. Myeloma alone were located at CD38strong positive (++) fractions. To further normal plasma mature phenotypically, we examined immunophenotypes of and by flow cytometry FITC-anti-CD38 phycoerythrin staining to VLA-4, MPC-1, CD44, CD56, CD19, CD20, CD24, or CD10. Normal all VLA-4+VLA-5+MPC-1+CD44+ CD19+CD56- marrows seven healthy donors, tonsils four patients chronic tonsillitis, a spleen one patient idiopathic thrombocytopenic purpura, lymph nodes two lymphadenitis, respectively. On hand, (12 20 cases), VLA-4+VLA-5+MPC-1+, CD19- most them CD56+, there no phenotype cases myelomas tested. Thus, as for expression CD19 various tissues are CD19+CD56-, whereas phenotype. According this finding, investigated CD56 on (CD38++ fractions) monoclonal gammopathy undetermined significance (MGUS). Both CD19-DC56+ found five MGUS tested, suggesting consists phenotypically cells. Therefore, it is reasoned phenotypic anti-CD19 anti-CD56 antibodies can malignant (myeloma cells), detect even premyeloma states.