Clathrin-Mediated Auxin Efflux and Maxima Regulate Hypocotyl Hook Formation and Light-Stimulated Hook Opening in Arabidopsis

摘要: The establishment of auxin maxima by PIN-FORMED 3 (PIN3)- and AUXIN RESISTANT 1/LIKE AUX1 (LAX) (AUX1/LAX3)-mediated transport is essential for hook formation in Arabidopsis hypocotyls. Until now, however, the underlying regulatory mechanism has remained poorly understood. Here, we show that loss function clathrin light chain CLC2 CLC3 genes enhanced thereby curvature, alleviated inhibitory effect overproduction on delayed blue light-stimulated reduction opening. Moreover, pharmacological experiments revealed curvature clc2 clc3 were sensitive to efflux inhibitors 1-naphthylphthalamic acid 2,3,5-triiodobenzoic but not influx inhibitor 1-naphthoxyacetic acid. Live-cell imaging analysis further uncovered impaired PIN3 endocytosis promoted its lateralization cortical cells did affect localization. Taken together, these results suggest regulates through modulating localization efflux, providing a novel integrates developmental signals environmental cues regulate plant skotomorphogenesis photomorphogenesis.