Sequence analysis of the cDNA encoding human liver glycogen phosphorylase reveals tissue-specific codon usage

摘要: We have cloned the cDNA encoding glycogen phosphorylase (1,4-alpha-D-glucan:orthophosphate alpha-D-glucosyl-transferase, EC 2.4.1.1) from human liver. Blot-hybridization analysis using a large fragment of to probe mRNA rabbit brain, muscle, and liver tissues shows preferential hybridization RNA. Determination entire nucleotide sequence message has allowed comparison with previously determined muscle sequence. Despite an amino acid identity 80%, two cDNAs exhibit remarkable divergence in G+C content. In sequence, 86% nucleotides at third codon position are either deoxyguanosine or deoxycytidine residues, while homolog figure is only 60%, resulting strikingly different pattern usage throughout most The appears represent evolutionary mosaic; segment N-terminal 80 acids contains greater than 90% position. A survey other published mammalian sequences reveals that data for phosphorylases reflects bias patterns coding general.