Studies on the mechanisms of translocation and termination

摘要: This chapter addresses two long-standing questions concerning ribosome structure and function: (i) How are the mRNA tRNAs moved through following formation of each peptide bond? (ii) how does recognition a stop codon result in hydrolysis peptidyl-tRNA? Not surprisingly, results from structural biology have played an important part formulating mechanistic models for both these processes. Although information is essential understanding detailed molecular mechanisms such processes, it itself insufficient establishing whether or not they correct. There already sufficient published examples false inferences based on structures to remind us that need be tested experimentally, preferably by diverse approaches. Key aspects standard translocation termination emerged observations — cryoEM reconstructions x-ray crystallography, respectively. Both been subjected experimental tests various kinds, process continues many laboratories. In first this chapter, we describe experiments using single-molecule bulk fluorescence methods examine relationship between intersubunit movement, hybrid-states binding tRNA a6nd translocation. second part, discuss model mechanism translation crystal complexes, some model.