Ischemic preconditioning protects intestine from prolonged ischemia

摘要: Ischemic preconditioning (IP), obtained by exposure to brief periods of vascular occlusion, improves organ tolerance prolonged ischemia. The aim this study was evaluate the effects IP on intestinal morphology. Forty rats were subjected sham surgery (n = 20, group I) or II) with a cycle ischemia/reperfusion (10-minute occlusion superior mesenteric artery [SMA], followed 10-minute reperfusion) before ischemia produced SMA (45 minutes). Five animals in each sacrificed 2, 12, 24, and 48 hours after reperfusion. Intestinal samples processed for light electron microscopy. A TUNEL assay performed detect apoptosis. Statistical analysis used Student t test Kaplan-Meier survival curves. overall mortality sham-operated 15%, while no II died (NS). Histological evaluation showed early detachment epithelial cells from villous stroma accompanied marked congestion edema. Successive morphological changes represented leukocyte infiltration, focal necrosis, villus denudation loss. Group significantly reduced inflammatory infiltrates lamina propria greater height compared I. maximum number apoptotic nuclei observed both groups, Following 2 reperfusion significantly, apoptosis at 12 (P <.05). Electron microscopy severe mitochondrial basement membrane damage. findings confirm that attenuates alterations are invariably present