Cloning of a complete cDNA encoding human aromatase: immunochemical identification and sequence analysis.
作者： Nobuhiro Harada
DOI: 10.1016/S0006-291X(88)80903-3
关键词: Clone (cell biology) 、 Peptide sequence 、 cDNA library 、 Nucleic acid sequence 、 Aromatase 、 Biochemistry 、 Molecular biology 、 Open reading frame 、 Sequence analysis 、 Biology 、 Complementary DNA
摘要: Summary A complete cDNA clone encoding a human aromatase was isolated from placental library in λgtll. An antibody to the polypeptide specified by prepared, and Western blot analysis inhibition experiments of activity confirmed identification as cDNA. The 3030 bp with two unique Eco RI sites contained 3′-noncoding region 1397 bp, an open reading frame 1509 503 amino acid residues, 5′-noncoding 124 bp. Analysis sequence comparison other forms cytochrome P-450 indicated that this enzyme is form superfamily.
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sci-hub.se 参考文章(11)
J.T. Kellis, L.E. Vickery, Purification and characterization of human placental aromatase cytochrome P-450. Journal of Biological Chemistry. ,vol. 262, pp. 4413- 4420 ,(1987) , 10.1016/S0021-9258(18)61364-X
C T Evans, C J Corbin, C T Saunders, J C Merrill, E R Simpson, C R Mendelson, Regulation of estrogen biosynthesis in human adipose stromal cells. Effects of dibutyryl cyclic AMP, epidermal growth factor, and phorbol esters on the synthesis of aromatase cytochrome P-450 Journal of Biological Chemistry. ,vol. 262, pp. 6914- 6920 ,(1987) , 10.1016/S0021-9258(18)48331-7
Liat TAN, Norio MUTO, Purification and reconstitution properties of human placental aromatase. A cytochrome P-450-type monooxygenase. FEBS Journal. ,vol. 156, pp. 243- 250 ,(1986) , 10.1111/J.1432-1033.1986.TB09574.X
D.D. Hagerman, Human placenta estrogen synthetase (aromatase) purified by affinity chromatography. Journal of Biological Chemistry. ,vol. 262, pp. 2398- 2400 ,(1987) , 10.1016/S0021-9258(18)61668-0
Nobuhiro Harada, Novel properties of human placental aromatase as cytochrome P-450: purification and characterization of a unique form of aromatase. Journal of Biochemistry. ,vol. 103, pp. 106- 113 ,(1988) , 10.1093/OXFORDJOURNALS.JBCHEM.A122213
Steven Henikoff, Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. ,vol. 28, pp. 351- 359 ,(1984) , 10.1016/0378-1119(84)90153-7
Yoshiyasu Yabusaki, Masatoshi Shimizu, Hiroko Murakami, Keiko Nakamura, Kenji Oeda, Hideo Ohkawa, Nucleotide sequence of a full-length cDNA coding for 3-methylcholanthrene-induced rat liver cytochrome P-450MC Nucleic Acids Research. ,vol. 12, pp. 2929- 2938 ,(1984) , 10.1093/NAR/12.6.2929
Carole R. Mendelson, Elizabeth E. Wright, Claudia T. Evans, John C. Porter, Evan R. Simpson, Preparation and characterization of polyclonal and monoclonal antibodies against human aromatase cytochrome P-450 (P-450AROM), and their use in its purification Archives of Biochemistry and Biophysics. ,vol. 243, pp. 480- 491 ,(1985) , 10.1016/0003-9861(85)90525-9
Shizuo Nakajin, Masato Shinoda, Peter F. Hall, Purification to homogeneity of aromatase from human placenta Biochemical and Biophysical Research Communications. ,vol. 134, pp. 704- 710 ,(1986) , 10.1016/S0006-291X(86)80477-6
DANIEL W. NEBERT, MILTON ADESNIK, MINOR J. COON, RONALD W. ESTABROOK, FRANK J. GONZALEZ, F. PETER GUENGERICH, IRWIN C. GUNSALUS, ERIC F. JOHNSON, BYRON KEMPER, WAYNE LEVIN, IAN R. PHILLIPS, RYO SATO, MICHAEL R. WATERMAN, The P450 gene superfamily: recommended nomenclature. DNA (Mary Ann Liebert, Inc.). ,vol. 6, pp. 1- 11 ,(1987) , 10.1089/DNA.1987.6.1