Interdisciplinary approach to cell-biomaterial interactions: biocompatibility and cell friendly characteristics of RKKP glass-ceramic coatings on titanium.

摘要: In this work, titanium (Ti) supports have been coated with glass–ceramic films for possible applications as biomedical implant materials in regenerative medicine. For the film preparation, a pulsed laser deposition (PLD) technique has applied. The RKKP material, used coating deposition, was sol–gel derived target of following composition: Ca-19.4, P-4.6, Si-17.2, O-43.5, Na-1.7, Mg-1.3, F-7.2, K-0.2, La-0.8, Ta-4.1 (all wt%). prepared coatings were compact and uniform, characterised by nanometric average surface roughness. biocompatibility cell-friendly properties material tested. Cell metabolic activity proliferation human colon carcinoma CaCo-2 cells seeded on showed same exponential trend found control plastic substrates. By phalloidin fluorescence analysis, no significant modifications actin distribution revealed grown films. Moreover, these high mRNA expression markers involved protein synthesis, differentiation, such villin (VIL1), alkaline phosphatase (ALP1), β-actin (β-ACT), Ki67 RPL34, recorded. conclusion, findings, first time, demonstrated that allows adhesion, growth differentiation cell line.