Photodynamic therapy with porphyrin and phthalocyanine sensitisation: Quantitative studies in normal rat liver

摘要: Selective sensitisation of malignant tumours to monochromatic light (photodynamic therapy, PDT) is a promising approach cancer treatment, but current sensitisers are unsatisfactory and the parameters controlling effects produced in normal neoplastic tissue poorly understood. To quantify relatively homogeneous organ, we carried out experiments livers rats following systemic with haematoporphyrin derivative (HpD) new sensitiser, sulphonated aluminium phthalocyanine (AlSPc) using from an Argon pumped tunable dye laser. Damage PDT (dominant at 100 mW laser power) could be distinguished that due local hyperthermia 400 mW). For both sensitisers, extent necrosis increased applied energy was abolished by occluding hepatic blood flow during therapy. With HpD, maximum only few hours between not detectable when this interval week. AlSPc, liver changed little times 1 h 1000 (6 weeks), declined slowly thereafter, matching amount AlSPc measurable alkali extraction, although prolonged photosensitisation seen muscle. Less cutaneous photosensitivity than HpD. easier produce handle has more appropriate strong absorption peak (at 675 nm) these results, warrants further study as photosensitiser for PDT.