Methods for purification of recombinant aav vectors
作者： Gina Nichols , Peter S. Gagnon , Pauline McLean Quigley Sheldon , Barbara A. Thorne
DOI:
关键词: Helper virus 、 Vaccination 、 Nucleic acid 、 Biology 、 Genetic enhancement 、 Recombinant DNA 、 Virology 、 Gene transfer 、 Virus 、 Cellular proteins
摘要: Provided herein are methods for the purification of recombinant adeno-associated virus (rAAV) vectors that can be used gene transfer and specifically therapy or vaccination. Recombinant AAV invention substantially free in-process impurities, including production components such as cellular nucleic acids, proteins, helper virus, media components.
lens.org 参考文章(32)
N. Muzyczka, Use of adeno-associated virus as a general transduction vector for mammalian cells. Current Topics in Microbiology and Immunology. ,vol. 158, pp. 97- 129 ,(1992) , 10.1007/978-3-642-75608-5_5
G. Subramanian, Production and purification Kluwer Academic/Plenum. ,(2004)
Rikkle Bolding Jensen, Frank Bech Nygaard, Method for purification of factor vii ,(2006)
X Xiao, J Li, R J Samulski, Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vector. Journal of Virology. ,vol. 70, pp. 8098- 8108 ,(1996) , 10.1128/JVI.70.11.8098-8108.1996
Charles R. Petrie, Charles Richardson, Bryan Steadman, Thomas R. Foubert, Thomas S. Vedvick, Virus like particle purification ,(2008)
Tsutomu Kawasaki, Makoto Niikura, Yuriko Kobayashi, Fundamental study of hydroxyapatite high-performance liquid chromatography Journal of Chromatography A. ,vol. 515, pp. 91- 123 ,(1990) , 10.1016/S0021-9673(01)89306-7
A Srivastava, E W Lusby, K I Berns, Nucleotide sequence and organization of the adeno-associated virus 2 genome. Journal of Virology. ,vol. 45, pp. 555- 564 ,(1983) , 10.1128/JVI.45.2.555-564.1983
Catherine E. O'Riordan, Alan E. Smith, Amy L. (Ericson) Helgerson, Purification of adenovirus and AAV ,(2005)
E Burova, E Ioffe, Chromatographic purification of recombinant adenoviral and adeno-associated viral vectors: methods and implications Gene Therapy. ,vol. 12, ,(2005) , 10.1038/SJ.GT.3302611
Ralph W. Paul, Dan Morris, Bruce W. Hess, Joseph Dunn, Robert W. Overell, Increased Viral Titer Through Concentration of Viral Harvests from Retroviral Packaging Lines Human Gene Therapy. ,vol. 4, pp. 609- 615 ,(1993) , 10.1089/HUM.1993.4.5-609