Tracer and electrical coupling of rat suprachiasmatic nucleus neurons.

摘要: Whole-cell recording from single neurons of the suprachiasmatic nucleus with an electrode containing tracer neurobiotin resulted in staining multiple 30% cases. Typically, one neuron was darkly stained dendritic processes and axon clearly visible while other were lightly stained. The darkly-stained cells identified as recorded tracer-coupled to five neurons. resting membrane potential, input conductance, capacitance, decay time constant maximum H-current amplitude not significantly different those showing coupling. Stimulation preoptic area activated antidromic action potential or all-or-none small slow inward current some when synaptic transmission blocked by a calcium-free/Mn2+ solution. did "collide" orthodromically spike suggesting that represents signal electrotonically-coupled neuron. In addition, frequency biphasic field currents neighbouring cell firing increased depolarization decreased hyperpolarization cell. These data demonstrate chemical electrical low-resistance coupling neurons, which could be important synthesizing circadian rhythm.