Investigation of the mechanisms of pulsed electric fields on inactivation of enzyme: lysozyme.

摘要: Lysozyme was selected as a model enzyme to investigate the effects of pulsed electric fields (PEF) on its activity and structure. The irreversible inactivation lysozyme in sodium phosphate buffer (10 mM, pH 6.2) induced by PEF at 35 kV/cm followed first-order when treatment time longer than 300 µs. Unfolding structure PEF, accompanied cleavage disulfide bonds self-association aggregation applied dosage higher critical level. correlated loss α-helix secondary relative residual PEF-treated close agreement with molar ellipticity 208 nm. Both PEF- heat-induced inactivations were alteration lysozyme, but heat inconsistent.