Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress.
作者： Z. Dastoor , J.L. Dreyer
关键词: Staurosporine 、 Biology 、 Apoptosis 、 Transfection 、 Oxidative stress 、 Fibroblast 、 Cell culture 、 Cell biology 、 Glyceraldehyde 3-phosphate dehydrogenase 、 MG132 、 Molecular biology
摘要: Recent studies indicating a role of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in apoptosis or oxidative stress has been reported. Using confocal laser-scanning microscopy, we have investigated the cellular distribution GAPDH central nervous system (CNS)-derived cells (neuroblastoma mNB41A3), non-CNS derived (R6 fibroblast) and an apoptosis-resistant Bcl2 overexpressing cell line (R6-Bcl2). Induction by staurosporine MG132 H(2)O(2) FeCN enhanced nuclear translocation endogenous all types, as detected immunocytochemistry. In apoptotic cells, expression is three times higher than non-apoptotic cells. Consistent with for apoptosis, overexpression GAPDH-green fluorescent protein (GAPDH-GFP) hybrid increased import GAPDH-GFP into transfected number made them more sensitive to agents that induce apoptosis. prevents untransfected but not overexpress GAPDH-GFP. Our observations indicate may play stress, probably related activity DNA repair enzyme carrier pro-apoptotic molecules.
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