Tretinoin reverses upregulation of matrix metalloproteinase‐13 in human keloid‐derived fibroblasts

摘要: Keloids are skin abnormalities that characterized by excessive deposition of collagen bundles in the dermis. Patients with keloids complain not only about their cosmetic appearance, but also continuous itching and/or tenderness associated chronic inflammation. Degradation extracellular matrix (ECM) may be upregulated, expansion into circumferential skin, and high metabolic activity keloid tissues due to increased metalloproteinase (MMP) activity. Based on these hypotheses, we examined differences expression MMP-1, MMP-8, MMP-13 between keloid-derived normal dermal fibroblasts. Since retinoids potent inhibitors MMPs treatment photoaged cancers, whether or tretinoin affects MMP The results real-time polymerase chain reaction ELISA demonstrated significant upregulation downregulation MMP-1 MMP-8 fibroblasts, at both mRNA protein levels. control group was significantly upregulated after addition tretinoin, whereas no change observed group. peak 12 h, while In contrast, remarkably elevated suppressed, suppression h changed. decrease contribute accumulation type I III tissues, this mechanism modulated molecular interaction MMP-13. Tretinoin appeared reverse abnormal profile such as markedly MMP-13, partly through inactivation AP-1 pathway. present suggest clinically useful improve inflammation seen prevent skin.