Evaluation of a prototype HCV NS5b assay for typing strains of hepatitis C virus isolated from Tunisian haemodialysis patients
作者： S.Ben Othman , A. Trabelsi , A. Monnet , N. Bouzgarrou , F. Grattard
DOI: 10.1016/J.JVIROMET.2004.03.002
关键词: NS5B 、 Hepacivirus 、 Biology 、 Virus 、 Flaviviridae 、 Hepatitis C virus 、 Typing 、 Genotyping 、 Virology 、 Microbiology
摘要: Hepatitis C virus (HCV) strains isolated from 68 haemodialysis Tunisian patients exhibiting chronic infection were genotyped targeting the NS5b region of HCV genome using a prototype assay developed by Bayer HealthCare-Diagnostics (TRUGENE HCV). The overall results compared to those obtained with another same company based on sequencing 5' non-coding 5'NC genotyping kit). All could be typed typing kit, but only 62 (91; 2%) assay. both methods exhibited pattern at type level: 57 1, 3 2, and 2 4. At subtype level, eight that gave undetermined kit successfully kit; additional discrepant results. agreement between two assays was 74.2% level. In conclusion, appears much more accurate than strains, especially in attending centres where distribution suggests frequent nosocomial transmissions.
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