Stability of nicotinamide adenine dinucleotide immobilized to cyanogen bromide activated agarose.

摘要: The stability of NAD(H) immobilized to a crosslinked agarose support (Sepharose(R)-4B) was examined in buffer solutions at pH 7.0 and 8.5. Specifically, this study investigated particle attrition ligand leakage rates from cyanogen bromide activated support. Particle did not occur under the experimental conditions. Ligand were found be first order concentration with two labile populations ligand. two-population model is consistent coupling chemistry, which results both an isourea imidocarbonate linkage. rate over time scale days, constants ranging 0.007 0.15 d(-1), depending on solution pH. (c) 1997 John Wiley & Sons, Inc.