Severe controlled cortical impact in rats: assessment of cerebral edema, blood flow, and contusion volume.

摘要: ABSTRACT Controlled cortical impact (CCI) is a contemporary model of experimental cerebral contusion. We examined the cerebrovascular and neuropathologic effects severe CCI in rats. The utility magnetic resonance imaging (MRI) for assessment contusion volume after was also established. Severe (3.0 mm depth, 4 m/sec velocity) to left (L) parietal cortex produced anesthetized (isoflurane/N2O/O2), intubated, mechanically ventilated male Sprague–Dawley rats (n = 58). Physiologic parameters were controlled. time coure alterations edema [L-R% brain water (% BW) 3-mm coronal sections through injured contralateral hemispheres, wet-dry weight] evaluated at 2 h, 24 48 7 days posttrauma. Local blood flow (lCBF, measured 8 structures each hemisphere by autoradiography) days. Contusion (measured histology image analysis) assessed 14 6 both MRI an...