DNA Aggregation Induced by Polyamines and Cobalthexamine
作者： Juan Pelta , Françoise Livolant , Jean-Louis Sikorav
关键词: Kinetics 、 Solubility 、 Precipitation (chemistry) 、 Sodium 、 A-DNA 、 Spermidine 、 Spermine 、 Chemistry 、 Chromatography 、 DNA 、 Biophysics
摘要: We have studied the precipitation of short DNA molecules by polycations spermidine, spermine, and cobalthexamine. The addition these cations to a solution leads first DNA; further resolubilizes pellet. multivalent salt concentration required for resolubilization is essentially independent (between 1 microM/ml mg/ml) monovalent cation present in (up 100 mM). aggregates are anisotropic; those obtained presence polyamines spermidine spermine generally contain cholesteric liquid crystalline phase that flows spontaneously. In contrast this never seen propose ability condense fluid structures an essential feature their biological functions.
sci-hub.se 参考文章(31)
Seymour S. Cohen, Introduction to the polyamines ,(1971)
Jonathan Widom, Robert L. Baldwin, Cation-induced toroidal condensation of DNA studies with Co3+(NH3)6. Journal of Molecular Biology. ,vol. 144, pp. 431- 453 ,(1980) , 10.1016/0022-2836(80)90330-7
Leonard C. Gosule, John A. Schellman, DNA condensation with polyamines: I. Spectroscopic studies Journal of Molecular Biology. ,vol. 121, pp. 311- 326 ,(1978) , 10.1016/0022-2836(78)90366-2
Youri Timsit, Dino Moras, Groove-backbone interaction in B-DNA : implication for DNA condensation and recombination Journal of Molecular Biology. ,vol. 221, pp. 919- 940 ,(1991) , 10.1016/0022-2836(91)80184-V
M A Krasnow, N R Cozzarelli, Catenation of DNA rings by topoisomerases. Mechanism of control by spermidine. Journal of Biological Chemistry. ,vol. 257, pp. 2687- 2693 ,(1982) , 10.1016/S0021-9258(18)34978-0
Barry Wolf, Stephen Berman, Sue Hanlon, Structural transitions of calf thymus DNA in concentrated LiCl solutions. Biochemistry. ,vol. 16, pp. 3655- 3662 ,(1977) , 10.1021/BI00635A023
D.C. Rau, V.A. Parsegian, Direct measurement of the intermolecular forces between counterion-condensed DNA double helices. Evidence for long range attractive hydration forces. Biophysical Journal. ,vol. 61, pp. 246- 259 ,(1992) , 10.1016/S0006-3495(92)81831-3
David E. Wemmer, Kalkunte S. Srivenugopal, Brian R. Reid, David R. Morris, Nuclear magnetic resonance studies of polyamine binding to a defined DNA sequence. Journal of Molecular Biology. ,vol. 185, pp. 457- 459 ,(1985) , 10.1016/0022-2836(85)90418-8
Juan A. Subirana, Juan L. Vives, The precipitation of DNA by spermine Biopolymers. ,vol. 20, pp. 2281- 2283 ,(1981) , 10.1002/BIP.1981.360201019
M. Olvera de la Cruz, L. Belloni, M. Delsanti, J. P. Dalbiez, O. Spalla, M. Drifford, Precipitation of highly charged polyelectrolyte solutions in the presence of multivalent salts Journal of Chemical Physics. ,vol. 103, pp. 5781- 5791 ,(1995) , 10.1063/1.470459