Chondroitin sulfate lyases: applications in analysis and glycobiology.

摘要: Publisher Summary This chapter discusses the structure characterization and active site chemistry of chondroitinases their implication for advancing knowledge on Chondroitin sulfate/dermatan sulfate (CS/DS) degradation mechanism. Chondroitinases constitute until now a valuable tool generation disaccharides indispensable fine CS analyses assisted by analytical techniques. Similarly, biological oligosaccharides affords tools study carbohydrate–protein interactions to development new therapeutic solutions. The use chondroitinase ABC treat central nervous system (CNS) injuries has proven be highly beneficial toward regeneration axons restoring synaptic plasticity in visual cortex. suggests exiting prospects these enzymes therapeutics CNS. DS are largely distributed as glycosaminoglycan (GAG) side chains proteoglycans (PG) found cellular membranes extracellular matrix (ECM) mammalian tissues. They known participate various physiological functions—for example, with proteins, activation growth factors, regulation angiogenesis, melanoma cell invasion proliferation.