IgG subclass distribution of anti-Rh, anti-Kell and anti-Duffy antibodies measured by sensitive haemagglutination assays.
作者： L Kornstad , T E Michaelsen
DOI:
关键词: Antibody 、 Isoantibodies 、 Molecular biology 、 Subclass 、 Antiserum 、 Isotype 、 Immunology 、 Hemagglutination 、 Immunoglobulin G 、 Rh blood group system 、 Virology 、 Biology
摘要: The IgG subclass distribution of anti Rh antibodies (anti-D, 'anti-Du', anti-c, anti-E), anti-Kell and anti-Duffy (anti-Fya) was measured by two haemagglutination techniques on microtitre plates. first technique involved rabbit specific antisera which were used to agglutinate red cells previously reacted with the patients' at high concentration. second, more sensitive, had an additional step introducing sheep anti-rabbit (sandwich technique). By sensitive sandwich we revealed, for anti-D antibodies: IgG1 8/19, IgG3 1/19, IgG1/IgG3 IgG1/IgG2/IgG3/IgG 41/19, IgG1/IgG4 1/19; Du reactive 1/8, IgG1/IgG3/IgG4 6/8; anti-E IgG1/IgG2/IgG4 2/3, IgG1/IgG2/IgG3/IgG4 1/3; anti-c 2/5, 1/5, 1/5; 9/20, 1/20, 8/20, 2/20; 7/8. These results are partly variance published results.
参考文章(22)
J.B. Natvig, H.G. Kunkel, Human immunoglobulins: classes, subclasses, genetic variants, and idiotypes. Advances in Immunology. ,vol. 16, pp. 1- 59 ,(1973) , 10.1016/S0065-2776(08)60295-3
C.G. Gahmberg, Molecular characterization of the human red cell Rho(D) antigen. The EMBO Journal. ,vol. 2, pp. 223- 227 ,(1983) , 10.1002/J.1460-2075.1983.TB01409.X
N. C. Hughes-Jones, The Estimation of the Concentration and Equilibrium Constant of Anti-D Immunology. ,vol. 12, pp. 223- 230 ,(1967) , 10.1007/978-94-011-6138-1_37
Blas Frangione, Terje E. Michaelsen, Edward C. Franklin, The Amino Acid Sequence of a Human Immunoglobulin G3m(g) pFc′ Fragment Journal of Immunology. ,vol. 119, pp. 558- 563 ,(1977)
D. E. Isenman, K. J. Dorrington, R. H. Painter, The structure and function of immunoglobulin domains. II. The importance of interchain disulfide bonds and the possible role of molecular flexibility in the interaction between immunoglobulin G and complement. Journal of Immunology. ,vol. 114, pp. 1726- 1729 ,(1975)
R C Aalberse, J van Leeuwen, R van der Gaag, Serologic aspects of IgG4 antibodies. I. Prolonged immunization results in an IgG4-restricted response. Journal of Immunology. ,vol. 130, pp. 722- 726 ,(1983)
L. Thorsteinsson, T.E. Michaelsen, S.S. Frøland, Specificity of Receptors for IgG on Human Cytotoxic Plaque-Forming Mononuclear Leukocytes International Archives of Allergy and Immunology. ,vol. 62, pp. 111- 117 ,(1980) , 10.1159/000232501
Terje E. Michaelsen, Eva Haug, Human IgG subclass-specific rabbit antisera suitable for immunoprecipitation in gel, ELISA and multilayer haemagglutination techniques. Journal of Immunological Methods. ,vol. 84, pp. 203- 220 ,(1985) , 10.1016/0022-1759(85)90428-4
Farouk Shakib, Denis R. Stanworth, Human IgG subclasses in health and disease. (A review). Part I. La Ricerca in Clinica E in Laboratorio. ,vol. 10, pp. 463- 479 ,(1980) , 10.1007/BF02938793
Royston Jefferis, M.Iskandar Nik Jaafar, Michael Steinitz, Immunogenic and antigenic epitopes of immunoglobulins. VIII. A human monoclonal rheumatoid factor having specificity for a discontinuous epitope determined by histidine/arginine interchange as residue 435 of immunoglobulin G Immunology Letters. ,vol. 7, pp. 191- 194 ,(1984) , 10.1016/0165-2478(84)90042-7