HIV-1 trafficking to the dendritic cell-T-cell infectious synapse uses a pathway of tetraspanin sorting to the immunological synapse

摘要: Dendritic cells (DCs) are essential components of the early events HIV infection. Here, we characterized trafficking pathways that HIV-1 follows during its capture by DCs and subsequent presentation to CD4(+) T via an infectious synapse. Immunofluorescence microscopy indicates virus-containing compartment in mature (mDCs) co-labels for tetraspanins CD81, CD82, CD9 but contains little CD63 or LAMP-1. Using ratio imaging pH-reporting fluorescent virions live DCs, show is internalized intracellular endocytic with a pH 6.2. Significantly, demonstrate infectivity cell-free virus more stable at mildly acidic than neutral pH. electron microscopy, confirm accumulates vacuoles contain CD81 positive internal membranes overlaps only partially CD63. When allowed contact cells, HIV-1-loaded redistribute CD9, as well HIV-1, not immunological synapse markers MHC-II T-cell receptor Together, our results indicate into non-conventional, non-lysosomal, mDCs further suggest able selectively subvert machinery required formation DC-T-cell facilitate own cell-to-cell transfer propagation.