Convenient chromatographic prepurification step before measurement of urinary cortisol by radioimmunoassay
作者： Gilles Morineau , James Gosling , Marie-Claude Patricot , Hany Soliman , Philippe Boudou
DOI: 10.1093/CLINCHEM/43.5.786
关键词: High-performance liquid chromatography 、 Chemistry 、 Sample preparation 、 Extraction (chemistry) 、 Gas chromatography–mass spectrometry 、 Gas chromatography 、 Chromatography 、 Urine 、 Sephadex 、 Isotope dilution
摘要: We applied various prepurification protocols (extraction with different solvents, liquid/solid separation on bonded silica media, Celite, and Sephadex LH20 chromatography) a range of commercially available RIA kits to measure cortisol in urine samples. then compared the results concentrations measured by HPLC method validated reference isotope dilution gas chromatography-mass spectrometry. conclude that chromatography commercial, prepacked diol minicolumn (Waters Sep-Pak Vac RC) combination dichloromethane extraction is convenient very effective purification step before urinary patients not receiving corticoid medication. tested numerous steroids for interference found free polar derivatives (hydroxylated or hydrogenated) could only partially account overestimations routinely encountered when are direct RIA.
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