The Cytoproct of Paramecium Caudatum: Structure and Function, Microtubules, and Fate of Food Vacuole Membranes

作者: R. W. Wolf , Richard D. Allen

DOI: 10.1242/JCS.14.3.611

关键词: Basal bodyVacuoleCytoplasmBiophysicsEndocytic cycleBiologyFood vacuoleAnatomyParamecium caudatumEndocytosisMicrotubule

摘要: The cytoproct or cell anus of Paramecium caudatum was studied, using light optics and electron microscopy, at known times before, during following food vacuole egestion. This accomplished by microscopically observing single cells, fixing these cells specific finally serial sectioning individually processed cells. cytoproct, rest, is a long narrow ridge along the posterior suture. It contains 2 uniquely positioned components which identify this structure as cytoproct: piles fibres inside surfaces ridge, microtubules passing from epiplasm summit down into cytoplasm. plasma membrane continuous over top ridge. cortical basal bodies adjacent to have bundles cytoplasm an opaque plaque their proximal ends. These sets may function in guiding vacuoles cytoproct. A model presented motive forces generated between bring and, addition, pull lips apart so that come contact fuse together, thus opening outside. increases area parting lips, possibly, result intercalation vesicles Immediately after formed changes smooth topography highly convoluted one. engulfed through process endocytosis resulting accumulation membranous fragments below endocytic probably about restitution pulling back together engulfed. filamentous meshwork underlying be active process.

参考文章(10)
Michael L. Watson, Staining of Tissue Sections for Electron Microscopy with Heavy Metals Journal of Cell Biology. ,vol. 4, pp. 475- 478 ,(1958) , 10.1083/JCB.4.4.475
Kathryn V. Holmes, Purnell W. Choppin, ON THE ROLE OF MICROTUBULES IN MOVEMENT AND ALIGNMENT OF NUCLEI IN VIRUS-INDUCED SYNCYTIA Journal of Cell Biology. ,vol. 39, pp. 526- 543 ,(1968) , 10.1083/JCB.39.3.526
Christian F. Bardele, A microtubule model for ingestion and transport in the suctorian tentacle Zeitschrift f�r Zellforschung und Mikroskopische Anatomie. ,vol. 126, pp. 116- 134 ,(1972) , 10.1007/BF00306784
Peter K. Hepler, J. Richard McIntosh, Story Cleland, Intermicrotubule bridges in mitotic spindle apparatus. Journal of Cell Biology. ,vol. 45, pp. 438- 444 ,(1970) , 10.1083/JCB.45.2.438
Edward S. Reynolds, THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY Journal of Cell Biology. ,vol. 17, pp. 208- 212 ,(1963) , 10.1083/JCB.17.1.208
JOHN M. MARSHALL, VIVIANNE T. NACHMIAS, CELL SURFACE AND PINOCYTOSIS. Journal of Histochemistry and Cytochemistry. ,vol. 13, pp. 92- 104 ,(1965) , 10.1177/13.2.92
On the Significance of Cross-Bridges Between Microtubules and Synaptic Vesicles Philosophical Transactions of the Royal Society B. ,vol. 261, pp. 395- 405 ,(1971) , 10.1098/RSTB.1971.0074
Foissner W, The cytopyge of ciliata. I. Its function, regeneration and morphogenesis in Uronema parduczi. Acta biologica Academiae Scientiarum Hungaricae. ,vol. 23, pp. 161- ,(1972)