Antimicrobial activity of eugenol and cinnamaldehyde against extended spectrum beta lactamase producing enterobacteriaceae by in vitro and molecular docking analysis

摘要: Abstract Introduction Bacterial resistance to third generation cephalosporins due Extended-Spectrum-Beta-Lactamase (ESBL) production is a major drug-resistance issue. The present work was undertaken investigate the antimicrobial activity of cinnamon, clove, garlic, tulsi and neem essential oils their bioactive compounds against pathogenic ESBL producing bacteria. Methodology property bacteria as well antibacterial effective compound(s) them investigated by disc diffusion assay. Minimum inhibitory concentrations (MIC) interaction with proteins were determined macro-broth dilution molecular docking method, respectively. Results Out 134 Enterobacteriaceae, 57.8% Escherichia coli 50.6% Klebsiella pneumoniae demonstrated property. Cinnamon clove oil exhibited stronger these isolates than tulsi, garlic or oil. Hence, main compounds, cinnamaldehyde eugenol, further tested identify properties. Approximately 86% 100% E. K. sensitive eugenol cinnamaldehyde, Eugenol MIC 499 μg/mL 63–999 μg/mL among maximum numbers , Cinnamaldehyde 122 μg/mL 245 μg/mL isolates, Lowest binding energies after predicted interactions them. These hydrogen bonded catalytic other crucial amino acid residues proteins. Conclusions Microbiological assays experiments indicated significant enzymes