Specific expression of N-acetylaspartate in neurons, oligodendrocyte-type-2 astrocyte progenitors, and immature oligodendrocytes in vitro.
作者： Jutta Urenjak , Stephen R. Williams , David G. Gadian , Mark Noble
DOI: 10.1111/J.1471-4159.1992.TB08875.X
关键词: Molecular biology 、 Galactocerebroside 、 Oligodendrocyte 、 Cerebellum 、 Neuron 、 Cell type 、 Biology 、 Progenitor cell 、 Astrocyte 、 Neuroglia 、 Biochemistry
摘要: To test the specificity of N-acetylaspartate (NAA) as a neuronal marker for proton nuclear magnetic resonance ( H-1 NMR) spectroscopy, purified and characterized cultured cells were analyzed their NAA content using both NMR HPLC. Cell types studied included cerebellar granule neurons, type-1 astrocytes, meningeal cells, oligodendrocyte-type-2 astrocyte (O-2A) progenitor oligodendrocytes. A high concentration was found in extracts neurons (approximately 12 nmol/mg protein), whereas remained undetectable mature However, twice level O-2A progenitors grown vitro. In addition significant levels also detected cultures immature Our data partly support previous suggestions that may be useful spectroscopic examination adult brain. they raise further possibility alterations associated with some specific brain disorders, particularly disorders seen newborn young children, reflect abnormalities development oligodendroglia or precursors.
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