Influence of cellular fatty acid composition on the response of Saccharomyces cerevisiae to hydrostatic pressure stress

摘要: High hydrostatic pressure (HHP) interferes with cellular membrane structure. The orientation of lipid molecules is changed, especially in the vicinity proteins, leading to decreased fluidity. Adaptation HHP requires increased fluidity, often achieved through a rise proportion unsaturated fatty acids. In this work, desaturase-deficient Saccharomyces cerevisiae mutant strain (OLE1 gene deletion) was grown media supplemented acids differing size and number unsaturations submitted up 200 MPa for 30 min. Desaturase-deficient yeast palmitoleic acid demonstrated sensitivity compared cells oleic or proportionate mixture both contrast, linoleic linolenic were more piezoresistant than treated acid. Furthermore, growth led higher levels peroxidation. Intracellular trehalose during treatment cell tolerance pressure. However, when remained extracellular sensitised Therefore, composition content might play role protection from oxidative damage produced by HHP, confirming that alteration fluidity correlated resistance yeast.