Transcriptional network profile on synovial fluid T cells in psoriatic arthritis

摘要: The objective of the study was to quantify transcriptional profile, as main T cell lineage-transcription factors on synovial fluid (SF) cells, in relation SF cytokines and frequencies (%) psoriatic arthritis (PsA) patients. Reverse phase protein array employed identify interleukin (IL)-23Rp19-, FOXP3- related orphan receptor gamma (RORγt)- Janus associated tyrosine kinases 1 (JAK1), signal transducer activator transcription (STAT1), STAT3 STAT5 phosphoproteins total lysates from PsA IL-1β, IL-2, IL-6, IL-21 interferon (INF)-γ were measured using a multiplex bead immunoassay patients peripheral blood (PB) healthy controls (HC). Frequencies CD4+CD25−, CD4+CD25high FOXP3+ CD127low Treg, either mean fluorescence intensity (MFI) CD4+ Treg or MFI classic IL-6 (IL-6R) α expression CD4+CD25− helper/effector cells (Th/eff) quantified PB HC by flow cytometry (FC). In samples, IFN-γ not detectable, whereas IL-1β levels higher than non-inflammatory osteoarthritis Higher IL-23R-, RORγt proteins JAK1, STAT1, found compared with HC. Direct correlations between JAK1 Y1022/Y1023 Y694, Y705 IL6, Increased proportion brighter IL-6Rα observed both CD4+CD25high- SF. showed distinctive JAK1/STAT3/STAT5 network joint microenvironment, outlining interplay IL-23, γC polarization plasticity Th17 which might participate perpetuation inflammation