PtdIns 3-Kinase Orchestrates Autophagosome Formation in Yeast
作者： Keisuke Obara , Yoshinori Ohsumi
DOI: 10.1155/2011/498768
关键词: Inner membrane 、 Cytoplasm 、 Biology 、 Phosphatidylinositol 、 Autophagosome 、 Cell biology 、 Biochemistry 、 Autophagy 、 Vacuole 、 Lysosome 、 Phosphorylation
摘要: Eukaryotic cells can massively transport their own cytoplasmic contents into a lytic compartment, the vacuole/lysosome, for recycling through conserved system called autophagy. The key process in autophagy is sequestration of within double-membrane structure, autophagosome. Autophagosome formation requires elaborate cooperation Atg (autophagy-related) proteins and lipid molecules. Phosphorylation phosphatidylinositol (PtdIns) by PtdIns 3-kinase, Vps34, step coordinating Vps34 forms two distinct protein complexes, only one which involved generating autophagic membranes. Upon induction autophagy, PtdIns(3)P, enzymatic product transported lumen vacuole via PtdIns(3)P enriched on inner membrane recruits Atg18−Atg2 complex presumably other to membranes, thereby molecules proteins.
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