Tissue growth into three‐dimensional composite scaffolds with controlled micro‐features and nanotopographical surfaces
作者： Elnaz Tamjid , Arash Simchi , John W. C. Dunlop , Peter Fratzl , Reza Bagheri
DOI: 10.1002/JBM.A.34584
关键词: Cell adhesion 、 Polycaprolactone 、 Materials science 、 Cellular differentiation 、 Extracellular matrix 、 Particle size 、 Biophysics 、 Bioactive glass 、 Tissue engineering 、 Nanoparticle 、 Biomedical engineering
摘要: Controlling topographic features at all length scales is of great importance for the interaction cells with tissue regenerative materials. We utilized an indirect three-dimensional printing method to fabricate polymeric scaffolds pre-defined and controlled external internal architecture that had interconnected structure macro- (400-500 μm) micro- (∼25 porosity. Polycaprolactone (PCL) was used as model system study kinetics growth within porous scaffolds. The surface decorated TiO2 bioactive glass (BG) nanoparticles better match nanoarchitecture extracellular matrix (ECM). Micrometric BG particles were also reveal effect particle size on cell behavior. Observation enzyme activity two-dimensional (2D) films (3D) showed effects nanoparticle inclusion curvature cellular adhesion, proliferation, preosteoblastic (MC3T3-E1) into pore channels. It found presence in substrate impaired adhesion proliferation 3D structures. Evaluation alkaline phosphate hard affects differentiation 2D films. Notwithstanding, not strong seen by substrate. observed different nanofeatures structures those scaffolds, which influence non-load-bearing applications bone medicine.
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