Cryoprotective leaf proteins.

摘要: Abstract Leaves of frost-resistant plants contain a number soluble proteins which are capable protecting isolated biomembranes against inactivation during freezing. Such have not been found in non-hardy summer material. The pattern protective was uniform hardy material different origin and appeared to change with the season. Cryoprotective were by preparative gel electrophoresis. Molecular weights as determined their electrophoretic mobility sodium dodecyl sulfate gels between 10 000 20 000. Circular dichroism measurements failed indicate helical structures. amino acid composition 2 active revealed high content polar acids. heat-stable. They were, on molar basis, more than 1000 times effective thylakoid membranes freezing damage low-molecular-weight cryoprotectants such sucrose, glycerol or dimethylsulfoxide. Very low concentrations increased cryoprotection provided sucrose. Of oligopeptides known composition, only few cryoprotective. Their activity very small compared that proteins. concentration cryoprotective leaves be enough for significant contribution frost tolerance resistant plants.