Host Vector System with the PR, Promoter of Phage Lambda
作者： N. I. Matvienko , I. N. Troyanovskaya , L. A. Zheleznaya , O. B. Yarchuk
DOI: 10.1007/978-94-011-6565-5_17
关键词: Cell biology 、 Transcription (biology) 、 Lambda phage 、 Promoter 、 Antitermination 、 Chemistry 、 Gene 、 Cosmid 、 Bacteriophage 、 Repressor
摘要: The early promoters of bacteriophage lambda (PL and PR) are widely used to regulate expression inserted genes. Despite the fact that PR, promoter is strongest transcription start site phage (1), vector molecules with this for foreign genes have not yet been constructed. This may be accounted by complex two-step process regulation from PR. promoter. Firstly inactivation repressor must occur allow PL N gene acts as a product antiterminator permitting extension into Q, whose protein product, in its turn, activates late lambda. It has proposed activation results antitermination 6S RNA, transcript permanently synthesized (1). Therefore order create host-vector system it necessary defective pro-phage includes cI, deletion
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