Dual effect of quercetin on rat isolated portal vein smooth muscle contractility.
作者： John A.O. Ojewole , Witness D.H. Chiwororo
DOI:
关键词: Prostacyclin 、 Nitric oxide synthase 、 Smooth muscle contraction 、 Medicine 、 Vasodilation 、 Vasoconstriction 、 Stimulation 、 Internal medicine 、 Prazosin 、 Endocrinology 、 Muscle contraction
摘要: This study examined the effects of quercetin on spontaneously contracting portal veins isolated from healthy young adult male and female Wistar rats (250-300 g). Quercetin (10(-7)-10(-4) M) always produced significant biphasic effects, comprising an initial brief stimulant effect (rise in basal tone), followed by a sustained, longer-lasting secondary relaxant (inhibitory) venous tissues. The contractions muscle preparations were not modified preincubation tissues with prazosin (10(-6) M), suggesting that upsurge tone increases contractile frequencies probably mediated via alpha1-adrenoceptor stimulation. However, nifedipine (10(-7) significantly suppressed (p 0.05) inhibited L-NAME (100 microM) or indomethacin (10 microM), vasorelaxant flavonoid was unlikely to be endothelium-dependent relaxing factor (EDRF), through prostacyclin (PGI(2)) pathways. N-p-tosyl-l-phenylalanine-chloromethyl-ketone (TPCK, 3 < 0.01) antagonised quercetin-induced relaxations, cAMP-dependent protein kinases might have contributed, at least part, towards rat veins.
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