Accurate characterization of porcine bivariate flow karyotype by PCR and fluorescence in situ hybridization

摘要: The 19 chromosomal pairs of the swine karyotype are resolved into 18 peaks denoted A to Q and Y by dual-beam flow cytometry. content six has previously been determined analyzing male/female differences, karyotypes animals carrying translocations, PCR studies genes with known assignments. For remaining chromosomes, putative assignments were deduced from comparison DNA contents, cytometry, size. We present here complete characterization pig bivariate using PARM-PCR technique combined fluorescence in situ hybridization. Chromosome-specific probes generated amplification 300 sorted chromosomes primers under nonspecific conditions used paint FISH. each peak was identified: (chromosome 1), B (13), C (6), D (2), E (14), F (3), G (7), H (9,4,X), H1 (9), I (15), J (8), K (5), L (10), M (12), N (16), O (11), P (17), (18), (Y). able characterize perfectly karyotype. Such techniques could be applied any other species.